ChromaCode is the result of taking a fresh perspective on an existing problem. The engineers and physicists at Caltech wanted to squeeze more performance out of traditional qPCR instruments. They realized that these instruments, though sophisticated, were only being used to answer simple questions such as is a gene present or not, is it at a high concentration or a low concentration?
They took a careful look at how PCR reactions worked. Realizing they could use chemistry to harness and shape the PCR curves themselves and taking inspiration from television data compression techniques, they developed a mathematical method to put more information into a single qPCR curve. They used the amplitude to unlock higher multiplexing in existing qPCR machines without modifying the hardware. High Definition PCR was born.
At ChromaCode, we leverage this novel perspective to develop highly multiplexed PCR assays. We are embedding this compression chemistry into a single HDPCR reagent and the decompression software into the intuitive cloud interface utilizing sophisticated signal processing, machine learning, and advanced analytics methods.
ChromaCode is deploying a disruptive solution for mid-density, multiplexed analysis, high-definition PCR or HDPCR, exclusively licensed from the California Institute of Technology. The technology is comprised of proprietary software and assay design that increases a qPCR instrument’s multiplexing capabilities. For example, rather than being restricted to one target per color channel, a 5-channel qPCR instrument could run 20-plex assays (4 x 5 colors) when combined with ChromaCode technology. This uniquely enables the production of midplex reagent panels and software that run on the existing qPCR instruments from large manufacturers globally without hardware modification.
As a result, HDPCR will enable the large and growing global qPCR instrument population (80,000+) to run midplex molecular panels for research applications.